{"product_id":"cst-80383s","title":"CST,  80383S, MDH1 (F5D2N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MDH1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MDH1 (F5D2N) Rabbit Monoclonal Antibody (CST #80383) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMDH1 (F5D2N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDH1 protein. This antibody does not cross-react with MDH2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro193 of human MDH1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMalate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate\/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate\/aspartate shuttle (1-3). The major function of the mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2). Studies show that MDH1 is part of the hydride transfer complex (HTC) in the cytoplasm that transfers reducing equivalents from NADH to NADP , reprogramming NAD metabolism and overcoming cellular senescence. HTC thus promotes tumor formation (4). In addition, it was shown that glycolytic activity increases in human breast cancer cells in early hypoxia independent of HIF1α activity. This enhanced glycolytic activity requires NAD provided by lactate dehydrogenase A (LDHA) and MDH1 that is fueled by glutamate-oxoglutarate transaminase 1 (GOT1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; EIEE88; epididymis secretory protein Li 32; HEL-S-32; malate dehydrogenase 1; malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal; MDH-s; MDH1; MDHA; MDHC; MGC:1375; MOR2; soluble malate dehydrogenase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799915417769,"sku":"80383S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80383s","provider":"Iright","version":"1.0","type":"link"}