{"product_id":"cst-80399s","title":"CST,  80399S, ING5 (F7J4V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ING5. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ING5 (F7J4V) Rabbit Monoclonal Antibody (CST #80399) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nING5 (F7J4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ING5 protein. This antibody does not cross-react with ING4 protein. This antibody detects bands of unknown origin between 80 and 160 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro223 of human ING5 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInhibitor of growth 5 (ING5) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators, which contain a conserved PHD domain (1,2). This protein family has been implicated in inhibition of cellular growth and is differentially expressed in cancer (3). ING5 associates with complexes containing HBO1 or H3-specific MOZ histone acetyltransferases (HATs) and is critical to histone acetylation associated with DNA replication during S-phase (4). ING5 associates with the TIP60 acetyltransferase to acetylate Lys120 of p53, promoting apoptosis (5). ING5 is phosphorylated on Thr152 by CDK2 in a cell cycle-dependent manner, and can also control apoptosis in a p53-independent manner (6). ING5 has been shown to be a positive prognostic marker in various cancer types (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ23842; ING5; inhibitor of growth family member 5; inhibitor of growth family, member 5; Inhibitor of growth protein 5; p28ING5\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799915450537,"sku":"80399S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80399s","provider":"Iright","version":"1.0","type":"link"}