{"product_id":"cst-80402t","title":"CST,  80402T, CLEC-1 (F2H2R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CLEC1A. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CLEC-1 (F2H2R) Rabbit Monoclonal Antibody (CST #80402) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:400\nFlow Cytometry (Live): 1:200 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCLEC-1 (F2H2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLEC-1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLEC-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nC-type lectin domain family 1 member A (CLEC-1) acts as a pattern recognition receptor for pathogens as a member of the C-type lectin superfamily (1). This protein family plays an important role in innate immunity and the adaptive immune response by stimulating cytokine secretion or T cell priming (1,2). CLEC-1 mRNA is primarily expressed in human endothelial and dendritic cells (DCs) and can be upregulated after stimulation with TGF-β (2,3). CLEC-1 localizes to the endoplasmic reticulum membrane fraction, showing a primarily intracellular localization and function (2,3). CLEC-1 may represent a target for cancer immunotherapy, as it has been shown to act as an immune checkpoint in myeloid cells (4). Absence of CLEC-1 reduced immunosuppressive myeloid cells in tumors and invigorated the activation state of DCs, thereby increasing T cell responses (4). Tripartite Motif Containing 21 (TRIM21) has been identified as a ligand for CLEC-1 (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC-type lectin domain family 1 member A; C-type lectin domain family 1, member A; C-type lectin-like receptor 1; C-type lectin-like receptor-1; CLC1A; CLEC-1; CLEC1; CLEC1A; MGC34328\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30-50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799915516073,"sku":"80402T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80402t","provider":"Iright","version":"1.0","type":"link"}