{"product_id":"cst-8050s","title":"CST,  8050S, Phospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate)","description":"Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate) (CST #8050) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:20\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS Buffer (pH 7.2), 0.1% Tween ® 20. Store at 4°C. Do not aliquot the antibodies.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Akt Substrate (RXXS*\/T*) (110B7E) Rabbit Monoclonal Antibody (Magnetic Bead Conjugate) recognizes peptides and proteins containing phospho-Ser\/Thr preceded by Arg at the -3 position. There is some preference observed for peptides that contain phospho-Ser\/Thr preceded by Arg at both positions -5 and -3. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides.\n\u003cb\u003eBackground\u003c\/b\u003e\nAn important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser\/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800800284841,"sku":"8050S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8050s","provider":"Iright","version":"1.0","type":"link"}