{"product_id":"cst-8068s","title":"CST,  8068S, Cleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GGA3 (Asp313) cleaved. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody (CST #8068) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved GGA3 (Asp313) (D16H3) Rabbit Monoclonal Antibody recognizes endogenous levels of GGA3 protein only when cleaved at Asp313.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp313 of human GGA3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer's Disease (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nADP-ribosylation factor-binding protein GGA3; GGA3; golgi associated, gamma adaptin ear containing, ARF binding protein 3; golgi-associated, gamma adaptin ear containing, ARF binding protein 3; Golgi-localized, gamma ear-containing, ARF-binding protein 3; KIAA0154\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799917449385,"sku":"8068S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8068s","provider":"Iright","version":"1.0","type":"link"}