{"product_id":"cst-80732s","title":"CST,  80732S, Phospho-IGF-I Receptor beta (Tyr1131)\/Insulin Receptor beta (Tyr1146) (D6D5L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IGF1R (Tyr1161) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IGF-I Receptor beta (Tyr1131)\/Insulin Receptor beta (Tyr1146) (D6D5L) Rabbit Monoclonal Antibody (CST #80732) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 40223 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IGF-I Receptor beta (Tyr1131)\/Insulin Receptor beta (Tyr1146) (D6D5L) Rabbit Monoclonal Antibody recognizes endogenous levels of IGF-I receptor β protein only when phosphorylated at Tyr1131 and\/or insulin receptor β protein only when phosphorylated at Tyr1146. This antibody cross-reacts with activated ErbB2 and ALK.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1131 of human IGF-I receptor protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nType I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146\/1150\/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD221; IGF-I receptor; IGF1R; IGFIR; IGFR; insulin like growth factor 1 receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799918006441,"sku":"80732S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80732s","provider":"Iright","version":"1.0","type":"link"}