{"product_id":"cst-80775s","title":"CST,  80775S, BACH2 (D3T3G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BACH2. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, BACH2 (D3T3G) Rabbit Monoclonal Antibody (CST #80775) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBACH2 (D3T3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total BACH2 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala304 of human BACH2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders, including type 1 diabetes, celiac disease, Crohn's disease, and the skin condition known as vitiligo (7-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBACH2; BTB and CNC homolog 2; BTB and CNC homology 1, basic leucine zipper transcription factor 2; BTB domain and CNC homolog 2; BTBD25; IMD60; Transcription regulator protein BACH2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799918563497,"sku":"80775S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80775s","provider":"Iright","version":"1.0","type":"link"}