{"product_id":"cst-80862s","title":"CST,  80862S, Ghost Dye UV 450 Fixable Viability Dye","description":"Cellular Dyes for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\n1. Prepare the following reagents with reverse osmosis deionized (RODI) or equivalent grade water: a. 1X PBS (azide- and protein\/serum-free) b. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C. ​​​​​​​ 2. Remove Ghost Dye from -20°C and bring to room temperature. ​​​​​​​ 3. Collect cells by centrifugation and aspirate supernatant. ​​​​​​​ 4. Wash cells by centrifugation in excess 1X PBS. Repeat if necessary. ​​​​​​​ 5. Resuspend cells to a concentration of 1-10 x 106\/mL in 1X PBS. ​​​​​​​ 6. Centrifuge the Ghost Dye before opening then add 1 uL for each 1 mL of cell suspension and vortex immediately. ​​​​​​​ 7. Incubate for 30 minutes at 4°C protected from light. ​​​​​​​ 8. Wash by centrifugation in excess incubation buffer. Discard supernatant. Repeat. ​​​​​​​ 9. Cells can then be fixed, permeabilized, and immunostained based upon experimental design and recommended protocols. ​​​​​​​ 10. Exclude cells with high Ghost Dye fluorescence from analysis. These were non-viable cells at the time of fixation. See details below for excitation and emission \u003cb\u003eSpecification\u003c\/b\u003es. ​​​​​​​ ​​​​​​​This product is excited by the UV (355 nm) laser line and has a peak emission of 450 nm that can be detected using a 450\/50 band pass filter commonly used for detection of DAPI, Hoechst 33258, etc.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C desiccated and protected from light. This product is stable for 12 months. Aliquot to avoid excessive freeze-thaw cycles.","brand":"CST","offers":[{"title":"Default Title","offer_id":46800742416553,"sku":"80862S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-80862s","provider":"Iright","version":"1.0","type":"link"}