{"product_id":"cst-81197s","title":"CST,  81197S, LAMP2A (F1A5A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying LAMP2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP2A (F1A5A) Rabbit Monoclonal Antibody (CST #81197) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunofluorescence (Frozen): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLAMP2A (F1A5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP2A protein. This antibody specifically detects the LAMP2A isoform (P13473-1) but not the LAMP2B (P13473-2) or LAMP2C (P13473-3) isoforms. Species reactivity by immunofluorescence is mouse only.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LAMP2A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Oxidation-induced activation of chaperone-mediated autophagy (CMA) is attained through transcriptional up-regulation of LAMP2A (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD107 antigen-like family member B; CD107b; DND; LAMP-2; LAMP2; LAMPB; LGP-96; LGP110; lysosomal associated membrane protein 2; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 100-130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799921578153,"sku":"81197S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-81197s","provider":"Iright","version":"1.0","type":"link"}