{"product_id":"cst-8178t","title":"CST,  8178T, C\/EBP alpha (D56F10) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying C\/EBP-alpha. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, C\/EBP alpha (D56F10) Rabbit Monoclonal Antibody (CST #8178) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200\nImmunofluorescence (Immunocytochemistry): 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46943 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nC\/EBP alpha (D56F10) Rabbit Monoclonal Antibody recognizes endogenous levels of total C\/EBPα protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human C\/EBPα protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCCAAT\/enhancer-binding proteins (C\/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C\/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C\/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C\/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C\/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\/EBP alpha; C\/EBP-alpha; CCAAT enhancer binding protein alpha; CCAAT\/enhancer binding protein (C\/EBP), alpha; CCAAT\/enhancer-binding protein alpha; CEBP; CEBPA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 42, 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799927378089,"sku":"8178T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8178t","provider":"Iright","version":"1.0","type":"link"}