{"product_id":"cst-82297t","title":"CST,  82297T, TCEB2\/Elongin B (F1K9D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TCEB2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TCEB2\/Elongin B (F1K9D) Rabbit Monoclonal Antibody (CST #82297) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTCEB2\/Elongin B (F1K9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCEB2\/Elongin B protein. Based on sequence homology, this antibody is not predicted to cross-react with TCEB1\/Elongin C protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCEB2\/Elongin B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTranscription elongation factor B polypeptide 2 (TCEB2), also known as Elongin B, is a regulatory component of the SIII\/Elongin elongation complex, which regulates elongation by RNA polymerase II by suppressing pausing of the polymerase during gene transcription. The SIII\/Elongin complex is also composed of TCEB3\/Elongin A, the catalytic subunit, and TCEB1\/Elongin C, another regulatory subunit (1). TCEB2\/Elongin B is part of the ubiquitin-proteasome pathway as a critical component of multiple E3 ligase complexes. It directly interacts with TCEB1\/Elongin C as part of the VHL complex, which targets HIF-1α, a transcription factor that plays a critical role in the cellular response to hypoxia, for degradation (2,3). Dysregulation of HIF-α activity is associated with poor prognosis in multiple human cancers (4). TCEB2\/Elongin B promotes therapy resistance in ovarian cancer (5,6), promotes breast cancer progression (7), and reduces radiosensitivity in triple-negative breast cancer (TNBC) (8). During HIV-1 infection, TCEB2\/Elongin B is part of an E3 ligase complex that targets the anti-viral proteins APOBEC3F and APOBEC3G for degradation (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEloB; Elongin 18 kDa subunit; elongin B; Elongin-B; elongin, 18-kD subunit; epididymis secretory sperm binding protein; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII; SIII p18; TCEB2; transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Transcription elongation factor B polypeptide 2; transcription elongation factor B subunit 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799930884265,"sku":"82297T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82297t","provider":"Iright","version":"1.0","type":"link"}