{"product_id":"cst-82318t","title":"CST,  82318T, BCKDK (E5B6J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BCKDK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BCKDK (E5B6J) Rabbit Monoclonal Antibody (CST #82318) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBCKDK (E5B6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCKDK protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCKDK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nBranched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Branched-chain α-keto acid dehydrogenase kinase (BCKDK) phosphorylates BCKDH-E1α (4). Phosphorylation of BCKDH-E1α plays a key role in regulating the enzymatic activity of this complex (3-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; 3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial; BCKD; BCKD-kinase; BCKDHKIN; BCKDK; BCKDKD; BDK; branched chain alpha-ketoacid dehydrogenase kinase; branched chain keto acid dehydrogenase kinase; branched chain ketoacid dehydrogenase kinase; Branched-chain alpha-ketoacid dehydrogenase kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799931310249,"sku":"82318T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82318t","provider":"Iright","version":"1.0","type":"link"}