{"product_id":"cst-82328sf","title":"CST,  82328SF, Phospho-IGF-I Receptor beta (Tyr1135\/1136)\/Insulin Receptor beta (Tyr1150\/1151) (19H7) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying InsR (Tyr1189\/Tyr1190) phosphate\/IGF1R (Tyr1165\/Tyr1166) phosphate. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-IGF-I Receptor beta (Tyr1135\/1136)\/Insulin Receptor beta (Tyr1150\/1151) (19H7) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #82328) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #3024. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3024\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IGF-I Receptor beta (Tyr1135\/1136)\/Insulin Receptor beta (Tyr1150\/1151) (19H7) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135\/1136 or tyrosine 1150\/1151, respectively. This antibody does not cross-react with other related tyrosine-phosphorylated tyrosine kinases.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1135\/1136 of human IGF-I receptor β.\n\u003cb\u003eBackground\u003c\/b\u003e\nType I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146\/1150\/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD220; CD221; HHF5; IGF-I receptor; IGF1R; IGFIR; IGFR; INSR; insulin like growth factor 1 receptor; Insulin receptor; Insulin receptor subunit alpha; Insulin receptor subunit beta; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; IR; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799931375785,"sku":"82328SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82328sf","provider":"Iright","version":"1.0","type":"link"}