{"product_id":"cst-82418t","title":"CST,  82418T, Tau (E5O2W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Tau mouse. Validated for Western Blotting,Simple Western™,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Tau (E5O2W) Rabbit Monoclonal Antibody (CST #82418) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunofluorescence (Frozen): 1:200 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79607 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTau (E5O2W) Rabbit Monoclonal Antibody recognizes endogenous levels of total tau protein. Nonspecific labeling of muscle in fixed frozen mouse colon has been observed by immunofluorescence.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of mouse tau protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI413597; AW045860; Mapt; Microtubule-associated protein tau; Mta; Mtapt; Neurofibrillary tangle protein; OTTMUSP00000003078; OTTMUSP00000003083; Paired helical filament-tau; PHF-tau; Tau\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 50-80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800129196201,"sku":"82418T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82418t","provider":"Iright","version":"1.0","type":"link"}