{"product_id":"cst-82457t","title":"CST,  82457T, RNASEH2A (F5D8B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RNASEH2A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RNASEH2A (F5D8B) Rabbit Monoclonal Antibody (CST #82457) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRNASEH2A (F5D8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total RNASEH2A protein. This antibody does not cross-react with RNASEH2B protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu289 of human RNASEH2A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe RNase H2 complex forms as a heterotrimer composed of RNASEH2A, RNASEH2B, and RNASEH2C. RNASEH2A is the catalytic component and is well conserved across species, while RNASEH2B and RNASEH2C are quite divergent (1). The RNase H2 complex is responsible for resolving DNA\/RNA hybrids, known as R-loops, which can occur during DNA replication and repair (1,2). The C-terminus of RNASEH2B is bound to PCNA, which then guides the complex to nuclear replication foci to resolve misincorporated ribonucleotides (2). Replicative stress leads to the increased expression of RNase H2 subunits, thereby reducing replication fork stalling in response to chemotherapeutics (3). Mutations in RNase H2 subunits cause Aicardi-Goutieres syndrome (AGS), a rare form of encephalopathy characterized by an increased innate immune response triggered by unresolved R-loops, mimicking a viral infection (4-6). RNASEH2B is frequently lost along with RB1 in advanced prostate cancer, which sensitizes cells to PARP and ATR inhibitors (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAGS4; Aicardi-Goutieres syndrome 4 protein; JUNB; Ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease H2, subunit A; Ribonuclease HI large subunit; Ribonuclease HI subunit A; ribonuclease HI, large subunit; RNase H(35); RNase H2 subunit A; RNase HI large subunit; RNASEH2A; RNASEHI; RNH2A; RNHIA; RNHL; THSD8\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799933145257,"sku":"82457T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82457t","provider":"Iright","version":"1.0","type":"link"}