{"product_id":"cst-82504t","title":"CST,  82504T, MDM2 (F7W7X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MDM2. Validated for WB,WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MDM2 (F7W7X) Rabbit Monoclonal Antibody (CST #82504) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62464 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMDM2 (F7W7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDM2 protein. Based on the sequence of the protein antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoforms A1, B, and D. By western blot, this antibody detects a band of unknown origin at approximately 60 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MDM2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nACTFS; Double minute 2 protein; double minute 2, human homolog of; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; LSKB; MDM2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog (mouse); MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; ubiquitin-protein ligase E3 Mdm2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799932883113,"sku":"82504T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82504t","provider":"Iright","version":"1.0","type":"link"}