{"product_id":"cst-82637s","title":"CST,  82637S, SRSF7 (F9G2P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SFRS7. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, SRSF7 (F9G2P) Rabbit Monoclonal Antibody (CST #82637) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSRSF7 (F9G2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRSF7 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect isoforms 1-4 of SRSF7.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro86 of human SRSF7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe serine\/arginine-rich family of splicing factors are crucial regulators in precursor RNA splicing, a process that is associated with maintenance of genome stability, regulation of mRNA export and stability, and translation (1). Serine\/arginine-rich splicing factor 7 (SRSF7\/SFRS7), also known as splicing factor 9G8, is overexpressed in colon and lung cancer, and regulates proliferation and apoptosis through the splicing of Fas mRNA in human colon and lung cancer cells (2). In human colon cancer cells, SRSF7 knockdown increased levels of the CDK inhibitor p21 Waf1\/Cip1 in a p53-independent manner (3). SRSF7 also regulates age-dependent splicing in mice (4) and induces expression of cellular senescence markers in response to DNA damage by generating an alternatively spliced form of p53, p53β (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n9G8; AAG3; aging-associated protein 3; serine and arginine rich splicing factor 7; Serine\/arginine-rich splicing factor 7; SFRS7; Splicing factor 9G8; Splicing factor, arginine\/serine-rich 7; splicing factor, arginine\/serine-rich 7, 35kDa; SR splicing factor 7; SRSF7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799933636777,"sku":"82637S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82637s","provider":"Iright","version":"1.0","type":"link"}