{"product_id":"cst-82846s","title":"CST,  82846S, Doublecortin (E3S7L) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)","description":"Monoclonal Antibody for studying doublecortin. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Doublecortin (E3S7L) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 488 Conjugate) (CST #82846) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunohistochemistry (Paraffin): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDoublecortin (E3S7L) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMutations in doublecortin ( ) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDBCN; DC; DCX; doublecortex; doublecortin; Doublin; Lis-X; Lissencephalin-X; LISX; Neuronal migration protein doublecortin; SCLH; XLIS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800540237993,"sku":"82846S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-82846s","provider":"Iright","version":"1.0","type":"link"}