{"product_id":"cst-83393s","title":"CST,  83393S, UBE2O Antibody","description":"Polyclonal Antibody for studying UBE2O. Validated for Western Blotting. Highly specific and rigorously validated in-house, UBE2O Antibody (CST #83393) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUBE2O Antibody recognizes endogenous levels of total UBE2O protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val576 of human UBE2O protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). UBE2O (E2-230K) is a unique member of the E2 family of ubiquitin-conjugating enzymes in that it functions as an E2-E3 hybrid enzyme (2). Research studies have demonstrated that UBE2O expression is regulated during erythroid differentiation, which suggests that its enzymatic activity participates in shaping the architecture of the erythrocyte proteome (3). UBE2O has also been implicated in BMP-7-induced adipocyte differentiation through its regulation of SMAD6 monoubiquitination (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n(E3-independent) E2 ubiquitin-conjugating enzyme; E2-230K; E2\/E3 hybrid ubiquitin-protein ligase UBE2O; FLJ12878; KIAA1734; UBE2O; Ubiquitin carrier protein O; ubiquitin conjugating enzyme E2 O; ubiquitin conjugating enzyme E2O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2O; Ubiquitin-protein ligase O\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799937142953,"sku":"83393S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-83393s","provider":"Iright","version":"1.0","type":"link"}