{"product_id":"cst-83565t","title":"CST,  83565T, GARP\/LRRC32 (E2L6B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GARP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GARP\/LRRC32 (E2L6B) Rabbit Monoclonal Antibody (CST #83565) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGARP\/LRRC32 (E2L6B) Rabbit Monoclonal Antibody recognizes endogenous levels of total GARP\/LRRC32 protein. This antibody cross-reacts with an unidentified protein of 32 kDa in some cell extracts.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala292 of human GARP\/LRRC32 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLeucine-rich repeat containing 32 (LRRC32), also known as glycoprotein A repetitions predominant (GARP), is a key regulator of the TGF-beta family (TGF-β1, TGF-β2, and TGF-β3), and is primarily expressed on the surface of mesenchymal stromal cells, hepatic stellate cells, platelets, and regulatory T cells (Tregs) (1). GARP\/LRRC32 enhances the suppressive function of Tregs and decreases the expression of inflammatory cytokines, IL-2, and IFNγ (2,3). GARP\/LRRC32 associates via disulfide bonds with the latency-associated peptide (LAP), the regulatory chain of TGF-β, to induce integrin-dependent activation of TGF-β and secretion (1, 3-5), thereby promoting Treg function and homeostasis. As a result, targeting GARP\/LRRC32 in combination with other molecules for depleting Tregs or attenuating their suppressive activity represents an opportunity for cancer immunotherapy (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nD11S833E; GARP; Garpin; Glycoprotein A repetitions predominant; leucine rich repeat containing 32; Leucine-rich repeat-containing protein 32; LRC32; LRRC32; Transforming growth factor beta activator LRRC32\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799938551977,"sku":"83565T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-83565t","provider":"Iright","version":"1.0","type":"link"}