{"product_id":"cst-83649t","title":"CST,  83649T, Connexin 43 (E7N2R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Connexin-43. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Connexin 43 (E7N2R) Rabbit Monoclonal Antibody (CST #83649) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nImmunofluorescence (Frozen): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 89289 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nConnexin 43 (E7N2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total connexin 43 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg374 of human connexin 43 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nConnexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAVSD3; CMDR; connexin 43; Connexin-43; Cx43; CXA1; DFNB38; EKVP; EKVP3; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction protein alpha 1; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha-like; GJA1; GJAL; HLHS1; HSS; ODDD; PPKCA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 39, 41, 43, 44\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799939141801,"sku":"83649T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-83649t","provider":"Iright","version":"1.0","type":"link"}