{"product_id":"cst-83669s","title":"CST,  83669S, TAZ (E8E9G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TAZ. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TAZ (E8E9G) Rabbit Monoclonal Antibody (CST #83669) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44080 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTAZ (E8E9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total TAZ protein. This antibody does not cross-react with YAP.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein corresponding to human TAZ protein. The epitope has been mapped to a region surrounding Pro49.\n\u003cb\u003eBackground\u003c\/b\u003e\nTAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ is negatively regulated via phosphorylation by LATS1\/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp586I1419; FLJ27004; FLJ45718; TAZ; transcriptional co-activator with PDZ-binding motif; Transcriptional coactivator with PDZ-binding motif; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; WWTR1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799939567785,"sku":"83669S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-83669s","provider":"Iright","version":"1.0","type":"link"}