{"product_id":"cst-8431s","title":"CST,  8431S, CISH (D4C10) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CISH. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CISH (D4C10) Rabbit Monoclonal Antibody (CST #8431) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCISH (D4C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total CISH protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human CISH protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak\/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. CISH\/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO (4). The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown (4). CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity (4-6). Expression of CISH is regulated by Stat5, thereby providing feedback modulation (5). Transgenic mice overexpressing CISH display phenotypes similar to Stat5 knockouts, including defects in mammary gland development and in T and NK cell regulation (6). Research studies have shown that polymorphisms within the CISH gene are associated with susceptibility to infectious diseases (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBACTS2; CIS; CIS-1; CISH; cytokine inducible SH2 containing protein; cytokine inducible SH2-containing protein; cytokine-inducible inhibitor of signaling type 1B; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 32, 37\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799941664937,"sku":"8431S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8431s","provider":"Iright","version":"1.0","type":"link"}