{"product_id":"cst-84393s","title":"CST,  84393S, CD86\/B7-2 (GL-1) Rat Monoclonal Antibody (APC Conjugate)","description":"Monoclonal Antibody for studying CD86 mouse. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD86\/B7-2 (GL-1) Rat Monoclonal Antibody (APC Conjugate) (CST #84393) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.06 μg of antibody per test.\nFlow Cytometry (Fixed\/Permeabilized): 1:330\nFlow Cytometry (Live): 1:330\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD86\/B7-2 (GL-1) Rat Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD86\/B7-2 protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9). The GL-1 antibody is used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivation B7-2 antigen; B7; B7-2; B7.2; B70; Cd28l; Cd28l2; Cd86; CD86 antigen; CLS1; early T cell costimulatory molecule-1; Early T-cell costimulatory molecule 1; ETC-1; Ly-5; Ly-58; Ly5; Ly58; MB7; MB7-2; OTTMUSP00000015940; T-lymphocyte activation antigen CD86; TS\/A-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2a kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800542236841,"sku":"84393S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-84393s","provider":"Iright","version":"1.0","type":"link"}