{"product_id":"cst-8461s","title":"CST,  8461S, Twinfilin-1 Antibody","description":"Polyclonal Antibody for studying A6. Validated for Western Blotting. Highly specific and rigorously validated in-house, Twinfilin-1 Antibody (CST #8461) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTwinfilin-1 Antibody recognizes endogenous levels of total twinfilin-1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human twinfilin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nTwinfilin is an actin monomer-binding protein found in all eukaryotes (1). Mammals have three isoforms. Twinfilin-1 and twinfilin-2a are expressed in most non-muscle cell types, whereas twinfilin-2b is the main isoform in adult heart and skeletal muscle (2). Twinfilins are composed of two ADF-homology domains connected by a 30 kDa linker region. All twinfilins have been shown to form a 1:1 complex with G-actin, but not F-actin (reviewed in 3). Twinfilin-1 was originally known as A6 protein tyrosine kinase and thought to be part of a novel class of protein kinases. However, the protein was renamed after further studies showed no evidence of tyrosine kinase activity (4). Twinfilin-1 helps to prevent the actin filament assembly by forming a complex with actin monomers and, in mammals, has been shown to cap the filament barbed ends. It has been suggested that this regulates cell motility (5). Suppression of twinfilin-1 has also been shown to slow lymphoma cell migration to lymph nodes (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nA6; A6 protein tyrosine kinase; MGC23788; MGC41876; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF1; twinfilin actin binding protein 1; Twinfilin-1; twinfilin, actin-binding protein, homolog 1; twinfilin, actin-binding protein, homolog 1 (Drosophila)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799945138345,"sku":"8461S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8461s","provider":"Iright","version":"1.0","type":"link"}