{"product_id":"cst-84671s","title":"CST,  84671S, SPAK (F7T1K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SPAK. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SPAK (F7T1K) Rabbit Monoclonal Antibody (CST #84671) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Frozen): 1:400 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSPAK (F7T1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPAK protein. Non-specific punctate cytoplasmic staining was observed in human kidney and staining of intercalated discs of human heart was observed by immunohistochemistry.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu429 of human SPAK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSPAK (STE20\/SPS1-related Pro\/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family of serine\/threonine kinases. Overexpression and studies demonstrate that SPAK is able to activate p38 MAP kinase, indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243\/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3,4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knockdown of SPAK dramatically reduces TCR\/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDCHT; DKFZp686K05124; PASK; proline-alanine-rich STE20-related kinase; serine threonine kinase 39 (STE20\/SPS1 homolog, yeast); serine\/threonine kinase 39; Serine\/threonine-protein kinase 39; small intestine SPAK-like kinase; SPAK; Ste-20-related kinase; Ste20-like protein kinase; STE20\/SPS1 homolog; STE20\/SPS1-related proline-alanine-rich protein kinase; STK39; STLK3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 65\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799945760937,"sku":"84671S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-84671s","provider":"Iright","version":"1.0","type":"link"}