{"product_id":"cst-8487s","title":"CST,  8487S, Sestrin-2 (D1B6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SESN2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Sestrin-2 (D1B6) Rabbit Monoclonal Antibody (CST #8487) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSestrin-2 (D1B6) Rabbit Monoclonal Antibody recognizes overexpressed levels of total sestrin-2 protein, as well as endogenous levels of sestrin-2 protein when expression is high.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human sestrin-2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Sestrin family of proteins, which comprises sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, plays an important role in regulating the cellular response to oxidative stress. Sestrin-1 and -2, both transcriptional targets of p53, have been shown to activate AMPK activity toward TSC2, ultimately resulting in the inhibition of mTOR (1). Thus, sestrin-1 and -2 link p53 signaling to the regulation of cellular growth (2). Sestrins have also been shown to function as modulators of cellular hydrogen peroxide concentration. Hydrogen peroxide is an important signaling molecule that is highly genotoxic and must therefore be tightly regulated (3). 2-Cys peroxiredoxins are metabolizing enzymes that maintain low cellular concentrations of hydrogen peroxide, but are hyperoxidized to an inactive state by a rapid increase in concentration (4) before quickly being reduced back to their active state by sestrins (5). This allows a hydrogen peroxide signal to temporarily circumvent the protective activity of 2-Cys peroxiredoxins, while minimizing oxidative damage.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp761M0212; DKFZp761M02121; Hi95; hypoxia induced gene 95; hypoxia-induced; Hypoxia-induced gene; SES2; SESN2; SEST2; sestrin 2; Sestrin-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 56\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799947137193,"sku":"8487S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8487s","provider":"Iright","version":"1.0","type":"link"}