{"product_id":"cst-85045s","title":"CST,  85045S, AIRE (F5F8X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying AIRE. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, AIRE (F5F8X) Rabbit Monoclonal Antibody (CST #85045) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAIRE (F5F8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total AIRE protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly240 of human AIRE protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAutoimmune disease is a significant health concern resulting from a breakdown in normal immune self-tolerance. Immune self-tolerance is regulated by a complex system that includes key cell types, regulatory molecules, cytokines, and intricate immune cell selection processes (1-3). The autoimmune regulator (AIRE) is a transcription factor first discovered in autoimmune disease patients with rare genetic mutations (4-6). AIRE has four distinct domains: a caspase recruitment domain (CARD), the SAND domain, and plant homeodomains 1 and 2 (PHD1 and PHD2) (2,3). AIRE plays a vital role in promoting self-tolerance in the thymus by regulating expression of common tissue-restricted peripheral self-antigens called tissue-restricted antigens (TSAs) (3). AIRE is primarily expressed by medullary thymic epithelial cells (mTECs), and coordinates expression of tissue-restricted proteins that are presented on major histocompatibility complex class I and II (MHC-I, MHC-II) and to developing T cells in the thymus (2,3). AIRE facilitates self-tolerance in other ways, such as regulating mTEC differentiation, controlling thymic dendritic cell accumulation, and developing regulatory T cells via XCL1 expression (3). AIRE regulates the production of CCR4 and CCR7 binding cytokines in mTECs and can alter thymocyte development and migration (2,3). In thymic B cells, AIRE is involved in self-antigen presentation for negative selection (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIRE; AIRE1; APECED; APECED protein; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; Autoimmune regulator; PGA1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 70-80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799948382377,"sku":"85045S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-85045s","provider":"Iright","version":"1.0","type":"link"}