{"product_id":"cst-8516l","title":"CST,  8516L, Phospho-Rb (Ser807\/811) (D20B12) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Rb (Ser807\/Ser811) phosphate. Validated for WB,WB,WB,IP,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Rb (Ser807\/811) (D20B12) Rabbit Monoclonal Antibody (CST #8516) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFluorescent Western: 1:1000\nSimple Western™: 1:10\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nImmunofluorescence (Frozen): 1:400 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #www.cellsignal.com\/products\/primary-antibodies\/phospho-rb-ser807-811-d20b12-xp-rabbit-mab-bsa-and-azide-free\/41359?_=1652117273177\u0026amp;Ntt=41359\u0026amp;tahead=true\"\u0026gt;41359.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Rb (Ser807\/811) (D20B12) XP\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807\/811 of human Rb protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4\/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK\/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nexon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799950315689,"sku":"8516L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8516l","provider":"Iright","version":"1.0","type":"link"}