{"product_id":"cst-85322t","title":"CST,  85322T, EED (E4L6E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying EED. Validated for WB,WB,IP,IHC,IF,F,ChIP,ChIP,C\u0026amp;R,C\u0026amp;T,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, EED (E4L6E) Rabbit Monoclonal Antibody (CST #85322) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:100\nCUT\u0026amp;Tag: 1:100\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53069 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEED (E4L6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total EED protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EED protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage \u003cb\u003eSpecification\u003c\/b\u003e, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Embryonic ectoderm development protein (EED) is a component of the PRC2 complex, which together with Ezh2 and SUZ12 is absolutely required for histone methyl-transferase activity (6). EED, SUZ12 and EZH2 are overexpressed in various types of cancer, including tumors of the colon, breast, prostate and ovary (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOGIS; EED; embryonic ectoderm development; Embryonic ectoderm development protein; hEED; Polycomb protein EED; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50-70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799951560873,"sku":"85322T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-85322t","provider":"Iright","version":"1.0","type":"link"}