{"product_id":"cst-85578s","title":"CST,  85578S, Phospho-CXCR4 (Ser324\/Ser325) Antibody","description":"Polyclonal Antibody for studying CXCR4 (Ser324) phosphate\/CXCR4 (Ser325) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CXCR4 (Ser324\/Ser325) Antibody (CST #85578) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-CXCR4 (Ser324\/Ser325) Antibody recognizes endogenous levels of CXCR4 protein only when phosphorylated at Ser324 and Ser325. This antibody does not cross-react with CXCR4 protein when phosphorylated at Ser339.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser324\/Ser325 of human CXCR4 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCXCR4 is a chemokine receptor that belongs to the G protein-coupled receptor family. It is activated by a small cytokine, CXCL12, also known as stromal cell derived factor 1 (SDF1) (1). The main function of CXCR4 is the mediation of the homing of progenitor cells in the bone marrow and their recruitment to sites of injury (2). More recently, CXCR4 has been studied as a potential therapeutic target in the context of autoimmune diseases (3), as well as cancer, as the receptor is involved in the regulation of migration, proliferation, and survival of cancer cells (4). CXCR4 is rapidly phosphorylated at Ser324 and Ser325 by protein kinase C and G protein-coupled receptor kinase 6 upon treatment of cells with CXCL12 (2). Phosphorylation of CXCR4 at Ser324\/Ser325 is critical to the ubiquitination and degradation of CXCR4 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC-X-C chemokine receptor type 4; C-X-C motif chemokine receptor 4; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; CXC-R4; CXCR-4; CXCR4; D2S201E; FB22; Fusin; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPY3R; NPYR; NPYRL; NPYY3R; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIM; WHIMS\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 42-50\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799953297577,"sku":"85578S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-85578s","provider":"Iright","version":"1.0","type":"link"}