{"product_id":"cst-85650sf","title":"CST,  85650SF, FoxP1 (D35D10) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying FOXP1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, FoxP1 (D35D10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #85650) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #4402. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4402\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFoxP1 (D35D10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total FoxP1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu584 of human FoxP1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nForkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for FoxP1 as a tumor suppressor (7). Alternatively, FoxP1 may also act as an oncogene as it is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n12CC4; FLJ23741; fork head-related protein like B; forkhead box P1; Forkhead box protein P1; FOXP1; glutamine-rich factor 1; hFKH1B; HSPC215; Mac-1-regulated forkhead; MFH; MGC12942; MGC88572; MGC99551; QRF1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 82-90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799954051241,"sku":"85650SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-85650sf","provider":"Iright","version":"1.0","type":"link"}