{"product_id":"cst-86513t","title":"CST,  86513T, NID1\/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Nidogen-1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, NID1\/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody (CST #86513) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNID1\/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NID1\/nidogen-1 protein. This antibody cross-reacts with a band of unknown origin at approximately 80 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1186 of human NID1\/nidogen-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAll extracellular basement membrane contains four major components: type IV collagen, nidogens, laminins, and perlecan (1). Nidogens are a family of highly conserved, sulfated glycoproteins which interact with the laminin 1 short arm chain, type IV collagen, and perlecan. This linking effect contributes to basement membrane formation (2,3). There are two isoforms in the nidogen family, nidogen-1, and nidogen-2. They have a similar domain structure consisting of three globular domains separated by a link region and a rod region (3). The functions of the two isoforms are complementary. Knockout of both genes results in defective basement membrane formation and perinatal lethality due to abnormal lung\/heart\/limb development (4,5). In lung cancer, colorectal cancer, and ovarian cancer, increased nidogen-1 release contributes to tumor progression by promoting proliferation and migration\/invasion, EMT, and metastasis (6-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nenactin; Entactin; epididymis secretory sperm binding protein; NID; NID-1; NID1; nidogen 1; Nidogen-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 150\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799961653417,"sku":"86513T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-86513t","provider":"Iright","version":"1.0","type":"link"}