{"product_id":"cst-86652s","title":"CST,  86652S, CUT\u0026RUN Assay Kit","description":"CUT\u0026amp;RUN Kit for studying in the research area.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nAll components in this kit are stable for at least 12 months when stored at the recommended temperature.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThe CUT\u0026amp;RUN Assay Kit can be utilized with any CUT\u0026amp;RUN-validated antibody to detect endogenous levels of protein-DNA interactions and histone modifications in mammalian cells (see Figures 1-6). The kit is compatible with multiple species of antibodies, including rabbit and mouse. The positive control Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit Monoclonal Antibody #9751 detects multiple species of tri-methyl histone H3 Lys4 protein, including human, mouse, rat, and monkey. Primer sets are included for the human (#7014) and mouse (#7015) positive control RPL30 gene locus; however, the use of other species with the kit requires the design of additional control primer sets.\n\u003cb\u003eBackground\u003c\/b\u003e\nLike the chromatin immunoprecipitation (ChIP) assay, Cleavage Under Targets \u0026amp; Release Using Nuclease (CUT\u0026amp;RUN) is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-4). This assay can be used either to identify multiple proteins bound to a single, specific genomic region (by using different antibodies), or conversely, to map the various genomic regions associated with a single protein of interest. In addition, the CUT\u0026amp;RUN assay can be used to define the spatial and temporal relationship of a particular protein-DNA interaction. For example, the CUT\u0026amp;RUN assay can be used to determine the specific order of recruitment of various protein factors to a gene promoter or to \"measure\" the relative amount of a particular histone modification across an entire gene locus during gene activation. In addition to histone proteins, the CUT\u0026amp;RUN assay can also be used to analyze binding of transcription factors and cofactors, DNA replication factors, and DNA repair proteins (Figures 1-6). CUT\u0026amp;RUN provides a rapid, robust, and true low cell number assay for detection of protein-DNA interactions in the cell. Unlike the ChIP assay, CUT\u0026amp;RUN is free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a much faster and more efficient method for enriching protein-DNA interactions and identifying target genes. CUT\u0026amp;RUN can be performed in less than one day, from cells or tissue to purified DNA, and has been shown to work with as few as 5,000-10,000 cells per assay (Figures 1-4). Instead of fragmenting all of the cellular chromatin as done in ChIP, CUT\u0026amp;RUN utilizes an antibody-targeted digestion of chromatin, resulting in much lower background signal than seen in the ChIP assay. As a result, CUT\u0026amp;RUN requires only 1\/10th of the sequencing depth that is required for ChIP-seq assays (1,2). Finally, the inclusion of yeast spike-in control DNA allows for accurate quantification and normalization, correcting for technical variations among samples during DNA purification, library preparation, and NGS steps.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCnR; Cut \u0026amp; Run; CUT \u0026amp; Tag; cut and run; Cut and Run; cut and tag; CUT\u0026amp;RUN; CUT\u0026amp;Tag; cutandrun; cutandtag; histone modification","brand":"CST","offers":[{"title":"Default Title","offer_id":46800805789865,"sku":"86652S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-86652s","provider":"Iright","version":"1.0","type":"link"}