{"product_id":"cst-87129s","title":"CST,  87129S, RHAMM\/CD168 (E7S4Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RHAMM\/CD168\/HMMR. Validated for WB,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, RHAMM\/CD168 (E7S4Y) Rabbit Monoclonal Antibody (CST #87129) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:50 - 1:200\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35364 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRHAMM\/CD168 (E7S4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total RHAMM\/CD168 protein. Non-specific staining was observed in kidney proximal tubules.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RHAMM\/CD168 protein. The antigenic peptide spans a region that is 100% conserved among the four isoforms of RHAMM\/CD168 reported in Uniprot.\n\u003cb\u003eBackground\u003c\/b\u003e\nReceptor for Hyaluronic acid-Mediated Motility (RHAMM, known also as CD168 or HMMR) was first identified as a putative receptor for hyaluronic acid (HA) that modulated HA-mediated cell motility (1). RHAMM\/CD168 is functionally similar to the HA receptor CD44; however in contrast to CD44, RHAMM\/CD168 does not contain a transmembrane domain or a signal peptide leader sequence, and so is not targeted exclusively to the cell membrane (1). RHAMM\/CD168 has multiple isoforms; some are reportedly exported to the cell membrane in response to signaling by growth factors and cytokines (e.g., TGF-β) (2, 3), whereas others have been implicated in intracellular functions including mitotic spindle regulation (4). Cell surface RHAMM\/CD168 is localized to membrane ruffles, consistent with proteins that regulate cell motility (1). Numerous research studies have reported that the expression of RHAMM\/CD168 is positively associated with cancer cell growth, motility and\/or metastasis (5-7), in addition to HA-mediated inflammation (8), suggesting the potential for therapeutic approaches that target HA-receptor mediated signaling (9,10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD168; HMMR; Hyaluronan mediated motility receptor; hyaluronan-mediated motility receptor (RHAMM); IHABP; intracellular hyaluronic acid binding protein; Intracellular hyaluronic acid-binding protein; MGC119494; MGC119495; Receptor for hyaluronan-mediated motility; RHAMM\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799967223977,"sku":"87129S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87129s","provider":"Iright","version":"1.0","type":"link"}