{"product_id":"cst-87162t","title":"CST,  87162T, PHF19 (E8P4D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PHF19. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PHF19 (E8P4D) Rabbit Monoclonal Antibody (CST #87162) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPHF19 (E8P4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHF19 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PHF19 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPHD finger protein 19 (PHF19), also known as polycomb-like protein 3 (PCL3), is a polycomb group protein that functions as an accessory subunit of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at Lys27 by the EZH2 methyltransferase (1). PHF19 recruits PRC2 to target genes by binding trimethylated histone H3 Lys36, a mark of active chromatin, via its Tudor domain (2-4). PHF19 associates with PRC2 and the histone H3 Lys36 demethylases NO66 and FBXL10, and is required to recruit PRC2 and NO66\/FBXL10 to stem cell genes during differentiation, resulting in PRC2-mediated trimethylation of histone H3 Lys27, loss of trimethylated histone H3 Lys36, and transcriptional silencing (2-4). Thus, PHF19 is critical for the proper transition of stem cell genes from the active to inactive state during differentiation of embryonic stem cells.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhPCL3; MGC131698; MGC149712; MGC149713; MGC23929; MTF2L1; PCL3; PHD finger protein 19; PHF19; polycomb like 3; polycomb-like 3; Polycomb-like protein 3; TDRD19B; tudor domain containing 19B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799968075945,"sku":"87162T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87162t","provider":"Iright","version":"1.0","type":"link"}