{"product_id":"cst-8723s","title":"CST,  8723S, Apaf-1 (D7G4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying APAF. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Apaf-1 (D7G4) Rabbit Monoclonal Antibody (CST #8723) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nApaf-1 (D7G4) Rabbit Monoclonal Antibody recognizes endogenous levels of total Apaf-1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Apaf-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nApoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAPAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 135\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799968731305,"sku":"8723S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8723s","provider":"Iright","version":"1.0","type":"link"}