{"product_id":"cst-87242s","title":"CST,  87242S, MGST1 (F6F5T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MGST1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MGST1 (F6F5T) Rabbit Monoclonal Antibody (CST #87242) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:250 - 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMGST1 (F6F5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total MGST1 protein. This antibody detects a 45 kDa band of unknown identity in some cell lines. This antibody is not recommended for immunohistochemical analysis of mouse tissues.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human MGST1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMicrosomal glutathione S-transferase 1 (MGST1) is a member of the membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG) family of enzymes that catalyze the reaction of glutathione with a wide range of electrophilic compounds (1-3). MGST1 is a homotrimer that can bind three molecules of glutathione and plays a role in protection from reactive electrophilic intermediates and oxidative stress (1-3). MGST1 is abundant in the liver and has a broad subcellular distribution with high levels in endoplasmic reticulum and mitochondrial membranes (1-3). Activation of MGST1 occurs by various mechanisms including exposure to sulfhydryl-reacting agents, thiol-disulfide interchange, proteolysis, and sulfhydryl oxidation to form dimers (3,4). MGST1 overexpression has been demonstrated in various cancers, and it may be a potential therapeutic target due to its role in inhibiting ferroptosis in cancer cells (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nglutathione S-transferase 12; GST12; MGC14525; MGST; MGST-I; MGST1; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799968862377,"sku":"87242S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87242s","provider":"Iright","version":"1.0","type":"link"}