{"product_id":"cst-87276s","title":"CST,  87276S, Iba1\/AIF-1 (E4O4W) Feline Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying AIF1\/Iba1. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Iba1\/AIF-1 (E4O4W) Feline Chimeric Monoclonal Antibody (CST #87276) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIba1\/AIF-1 (E4O4W) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total Iba1\/AIF-1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from Iba1\/AIF-1 (E4O4W) XP  Rabbit mAb #17198 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Iba1\/AIF-1 (E4O4W) XP  Rabbit mAb #17198, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala139 of human Iba1\/AIF-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nIonized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1\/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1\/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1\/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial\/macrophage states (5). Iba1\/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia\/macrophages in the brain and other tissue (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Feline chimeric IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800301228201,"sku":"87276S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87276s","provider":"Iright","version":"1.0","type":"link"}