{"product_id":"cst-8743s","title":"CST,  8743S, Neurofilament-L (C28E10) Rabbit Monoclonal Antibody (Alexa Fluor® 594 Conjugate)","description":"Monoclonal Antibody for studying NFL. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Neurofilament-L (C28E10) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 594 Conjugate) (CST #8743) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNeurofilament-L (C28E10) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; FLJ53642; light molecular weight neurofilament protein; NEFL; Neurofilament L; neurofilament light; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament-light; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; NF-L; NF68; NFL; PPP1R110; protein phosphatase 1, regulatory subunit 110\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800548954281,"sku":"8743S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8743s","provider":"Iright","version":"1.0","type":"link"}