{"product_id":"cst-87592s","title":"CST,  87592S, TRIB1 (E6M3T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Tribbles1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TRIB1 (E6M3T) Rabbit Monoclonal Antibody (CST #87592) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTRIB1 (E6M3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIB1 protein. This antibody is not predicted to cross-react with other TRIBBLES proteins, based on sequence divergence in the epitope region.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRIB1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTRIBBLES proteins belong to a small family of serine-threonine kinase-like proteins characterized by the presence of a variant protein kinase motif (lacking a canonical ATP binding site), a MEK1 binding site, and a C-terminal COP1 site that binds ubiquitin ligase. The gene was first identified and characterized in genetic screens for genes that regulate cell division, gastrulation, and oogenesis (1-3). Research studies in suggested that TRIBBLES functions to coordinate cell division by regulating turnover of the cell cycle protein String\/cdc25. In contrast to the genome, which contains a single gene, the genomes of mice and humans encode three known TRIBBLES proteins (TRIB1-3), which exhibit both distinct and overlapping patterns of expression and functions (4). For example, TRIB1 and TRIB2, but not TRIB3, were reported to promote degradation of the basic region-leucine zipper transcription factor C\/EBPα, a function that appears to be conserved from flies to humans (5,6). TRIB2 is overexpressed in a subset of human AML patient samples, downregulated in leukemic cells undergoing proliferation arrest (7), and positively regulated by the NOTCH signaling pathway in T cells (8), while retroviral-mediated overexpression of TRIB2 in mice was shown to induce transplantable leukemia (7). These findings collectively suggest that TRIB2 functions as an oncogene in the mammalian hematopoietic system (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC8FW; G-protein-coupled receptor induced protein; G-protein-coupled receptor-induced gene 2 protein; G-protein-coupled receptor-induced protein 2; GIG-2; GIG2; phosphoprotein regulated by mitogenic pathways; SKIP1; TRB-1; TRB1; TRIB1; Tribbles homolog 1; tribbles homolog 1 (Drosophila); tribbles pseudokinase 1; tribbles-like protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799971352745,"sku":"87592S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87592s","provider":"Iright","version":"1.0","type":"link"}