{"product_id":"cst-87795t","title":"CST,  87795T, NEK7 (E4E1B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NEK7. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NEK7 (E4E1B) Rabbit Monoclonal Antibody (CST #87795) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNEK7 (E4E1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total NEK7 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NEK7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNEK7 (NIMA-related protein kinase 7), is one of a family of serine\/threonine kinases, named NEK1-NEK11, which are involved in the control of mitosis and other non-mitotic functions (1-6). NEK7 is the smallest NIMA-related kinase in mammals, and is widely expressed by many tissues (1-3). Its role in mitosis relates to centrosome enrichment and microtubule nucleation, and similar to other NIMA-related kinases, NEK7 is localized to the microtubule-organizing center (MTOC) at the time of mitotic entry (3,6). NEK7 has also been demonstrated to be essential for NLRP3 inflammasome activation by directly binding to the leucine-rich repeat (LRR) domain of NLRP3 (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nNEK7; Never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7; NIMA related kinase 7; NimA-related protein kinase 7; Serine\/threonine-protein kinase Nek7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799973253289,"sku":"87795T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-87795t","provider":"Iright","version":"1.0","type":"link"}