{"product_id":"cst-88086s","title":"CST,  88086S, SARS-CoV-2 Nsp3 Antibody","description":"Polyclonal Antibody for studying nsp3-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp3 Antibody (CST #88086) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-2 Nsp3 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp3 protein in infected cells.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro395 of SARS-CoV-2 Nsp3 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp3, also known as papain-like protease (PLpro), is the third protein to be translated from the viral RNA by the eukaryotic translational machinery. In a sequence of processing events, Nsp3 is cleaved from pp1a and pp1ab polyproteins, and works to also release 3C-like protease, a cysteine-like protease that performs subsequent polyprotein cleavage events that give rise to the components of the viral replicase complex (3). Both proteases are targets of interest for pharmaceutical anti-viral therapy (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nnon-structural protein 3; non-structural protein 3 (819-2763); nsp3; nsp3-pp1a-pp1ab; Papain-like proteinase; PL-PRO; PL2-PRO; PLPro; SARS coronavirus main proteinase; SARS-CoV-2 nsp3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Endogenous\nMW (kDa): 150-250\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800245260457,"sku":"88086S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88086s","provider":"Iright","version":"1.0","type":"link"}