{"product_id":"cst-88113s","title":"CST,  88113S, VCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying VCre recombinase. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, VCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody (CST #88113) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody recognizes transfected VCre recombinase protein. This antibody also detects a small subset of Cre positive cells by immunofluorescence.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of VCre recombinase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre\/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). Similar to Cre\/Lox, two additional site-specific recombination systems have been identified: VCre\/VloxP and SCre\/SloxP. Although the structure of their recognition sites are identical, Cre, VCre, and SCre recombinases do not cross-react with each other, allowing for in tandem use (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nphage integrase family protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800236740777,"sku":"88113S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88113s","provider":"Iright","version":"1.0","type":"link"}