{"product_id":"cst-8814t","title":"CST,  8814T, Non-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) (D13A1) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Catenin-beta (Ser33\/Ser37\/Thr41) non-phosphate. Validated for WB,IP,IHC,IF,F,ChIP,ChIP,C\u0026amp;R. Available in 3 sizes. Highly specific and rigorously validated in-house, Non-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) (D13A1) Rabbit Monoclonal Antibody (CST #8814) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:800 - 1:3200\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33893 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNon-phospho (Active) beta-Catenin (Ser33\/37\/Thr41) (D13A1) Rabbit Monoclonal Antibody recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. This antibody does not detect β-catenin protein if tri-phosphorylated at Ser33\/Ser37\/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nβ-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Non-phospho (Active) β-Catenin (Ser33\/37\/Thr41) (D13A1) Rabbit mAb is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and\/or the canonical Wnt signaling pathway.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\narmadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 92\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799978332329,"sku":"8814T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8814t","provider":"Iright","version":"1.0","type":"link"}