{"product_id":"cst-88197v2","title":"CST,  88197V2, FastScan™ LbCpf1 (Strain ND2006)\/Cas12a ELISA Kit","description":"FastScan ELISA Kit for studying LbCpf1 (Strain ND2006) in the research area.\n\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: ELISA+\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThe FastScan™ LbCpf1 (Strain\nSpecies Reactivity: All Species Expected\n\u003cb\u003eBackground\u003c\/b\u003e\nCRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1\/Cas12a (CRISPR from and ) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1\/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1\/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1\/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1\/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1\/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).~LbCpf1 (Strain )\/Cas12a is a Cpf1\/Cas12a enzyme derived from (2,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCpf1; LbCpf1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All","brand":"CST","offers":[{"title":"Default Title","offer_id":46800682385577,"sku":"88197V2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88197v2","provider":"Iright","version":"1.0","type":"link"}