{"product_id":"cst-8822s","title":"CST,  8822S, Nanog (D2A3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Nanog mouse. Validated for WB,IP,IF,F,ChIP,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, Nanog (D2A3) Rabbit Monoclonal Antibody (CST #8822) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:1000\nFlow Cytometry (Fixed\/Permeabilized): 1:200\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75840 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNanog (D2A3) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse nanog protein. This antibody is expected to recognize nanog 1a and 1b isoforms. This antibody does not cross-react with human nanog.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2410002E02Rik; early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ecat; ecat4; ENK; ES cell-associated protein 4; Homeobox protein NANOG; homeobox transcription factor Nanog; Nanog; Nanog homeobox; Q5TM83; Q80Z64; Q80Z64-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 29-42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800140992681,"sku":"8822S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8822s","provider":"Iright","version":"1.0","type":"link"}