{"product_id":"cst-88251s","title":"CST,  88251S, CD16 (2H7) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying CD16\/FCgR3a. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD16 (2H7) Mouse Monoclonal Antibody (CST #88251) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nIHC Leica Bond: 1:200 - 1:800\nImmunohistochemistry (Paraffin): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: IHC Leica Bond, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD16 (2H7) Mouse Monoclonal Antibody recognizes endogenous levels of CD16 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to the external domain of CD16 protein, common to both the transmembrane form and the GPI-linked form.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD16; CD16A; CD16a antigen; Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc gamma receptor III-A; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIIIa; FCG3; FCG3A; FcgammaRIIIA; FCGR3; FCGR3A; FCGRIII; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800288612521,"sku":"88251S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88251s","provider":"Iright","version":"1.0","type":"link"}