{"product_id":"cst-88274s","title":"CST,  88274S, MHC Class I (EMR8-5) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying HLAA\/HLAC\/HLAB. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, MHC Class I (EMR8-5) Mouse Monoclonal Antibody (CST #88274) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28149 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMHC Class I (EMR8-5) Mouse Monoclonal Antibody detects HLA-A, HLA-B, HLA-C, and HLA-E. This antibody does not cross-react with HLA-G.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with human HLA-A*2402 extracellular domain recombinant protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8 T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4 T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAS; B-4901; D6S204; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA-A; HLA-B; HLA-C; HLA-C antigen; HLA-Cw; HLA-JY3; HLAA; HLAB; HLAB iso9; HLAC; HLC-C; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leukocyte antigen class I-A; leukocyte antigen class I-B; major histocompatibility antigen HLA-C; major histocompatibility complex, class I, A; major histocompatibility complex, class I, B; major histocompatibility complex, class I, C; MHC; MHC class 1 antigen; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; PSORS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30-50\nSource\/Isotype: Mouse IgG1 kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46799980200105,"sku":"88274S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88274s","provider":"Iright","version":"1.0","type":"link"}