{"product_id":"cst-88358s","title":"CST,  88358S, Virilizer (D4N8B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Virilizer. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Virilizer (D4N8B) Rabbit Monoclonal Antibody (CST #88358) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVirilizer (D4N8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total virilizer protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro229 of human virilizer protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMethyltransferase-like protein 3 (METTL3) and methyltransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3). In this complex, METTL3 functions as the catalytic methyltransferase subunit and METTL14 functions as the target recognition subunit by binding to RNA (4). In addition, the Wilms' tumor 1-associating protein (WTAP) functions as a regulatory subunit and is required for accumulation of the complex to nuclear speckles, which are sites of RNA processing (5). Several studies suggest a role for this complex in cancer. METTL3 expression is elevated in lung adenocarcinoma where it promotes growth, survival, and invasion of human lung cancer cells (6). In addition, WTAP is overexpressed in a number of different cancers and positively regulates cell migration and invasion in glioblastoma and cholangiocarcinoma (7,8). Virilizer is an additional protein subunit of the METTL3\/METTL14 N6-methyltransferase complex and is required for methyltransferase activity (9). As part of the WTAP complex, virilizer functions to regulate sex determination in Drosophila melanogaster through splicing of Sex-lethal (Sxl) mRNA transcripts (10,11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp434I116; DKFZp781B2117; fSAP121; functional spliceosome-associated protein 121; KIAA1429; MGC138493; MGC141940; MSTP054; Protein virilizer homolog; VIR; vir like m6A methyltransferase associated; virilizer homolog; VIRMA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 210\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799980593321,"sku":"88358S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-88358s","provider":"Iright","version":"1.0","type":"link"}